Abstract: To explore soil fungal diversity in the Zhaling Lake and screen the antifungal strains against crop pathogens, and provide basic materials for subsequent development of biocontrol agents, 11 sediment samples were collected from shoreside of the Zhaling Lake. After being cultured on 7 selective mediums, the fungi were isolated by dilution separation method. The strains were identified by a combination of morphological and molecular biology methods, and the antifungal strains were screened by confront culture method. The results showed that a total of 100 fungi were isolated, belonging to 27 genera including Talaromyces, Alternaria, Cladosporium, Psiloglonium, Fusarium, Phom, Penicillium, Emericellopsis, Epicoccum, Trichurus and Stagonosporopsis etc. Among them, the genera Talaromyces (18 strains) and Alternaria (11 strains) were the dominant genera, and Schizophyllum, Podosordadia, Dothiorella, Radulidium, Trametes, Athelia, Coriolopsis, Geosmithia, Stemphylium, Bipolaris and Aspergillus were the rare genera with only 1 strain. Four highly active antifungal strains were screened belonging to Cladosporium, Emericellopsis, Truncatella and Athelia, they had better inhibitory effect on 8 fungi pathogens isolated from main crops in Qinghai Province. The soil fungi resources in the Zhaling Lake are abundant and the exploitation and utilization values are well.